LymphedemaV1, Main, Exploration, bibRecord, 006415

Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle

Identifieur interne : 006415 ( Main/Exploration ); précédent : 006414; suivant : 006416

Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle

Auteurs : Andrey Anisimov [Finlande] ; Annamari Alitalo [Finlande] ; Petra Korpisalo [Finlande] ; Jarkko Soronen [Finlande] ; Seppo Kaijalainen [Finlande] ; Veli-Matti Lepp Nen [Finlande] ; Michael Jeltsch [Finlande] ; Seppo Yl Herttuala [Finlande] ; Kari Alitalo [Finlande]

Source :

Circulation research [ 0009-7330 ] ; 2009.

RBID : PMC:2776655

Descripteurs français

English descriptors

KwdEn :
- Animals, Blood Vessels (physiology), Dimerization, Drug Stability, Heart (physiology), Humans, Lymphatic Vessels (physiology), Mice, Mice, Transgenic, Muscle, Skeletal (blood supply), Muscle, Skeletal (physiology), Mutation, Polymorphism, Single Nucleotide, Recombinant Proteins (metabolism), Vascular Endothelial Growth Factor B (genetics), Vascular Endothelial Growth Factor B (physiology), Vascular Endothelial Growth Factor C (genetics), Vascular Endothelial Growth Factor C (physiology), Vascular Endothelial Growth Factor Receptor-2 (physiology).
MESH :
- chemical , genetics : Vascular Endothelial Growth Factor B, Vascular Endothelial Growth Factor C.
- chemical , metabolism : Recombinant Proteins.
- blood supply : Muscle, Skeletal.
- physiology : Blood Vessels, Heart, Lymphatic Vessels, Muscle, Skeletal, Vascular Endothelial Growth Factor B, Vascular Endothelial Growth Factor C, Vascular Endothelial Growth Factor Receptor-2.
- Animals, Dimerization, Drug Stability, Humans, Mice, Mice, Transgenic, Mutation, Polymorphism, Single Nucleotide.

Abstract

The therapeutic potential of vascular endothelial growth factor C (VEGF-C) and VEGF-D in skeletal muscle has been of considerable interest as these factors have both angiogenic and lymphangiogenic activities. Previous studies have mainly employed adenoviral gene delivery for short-term expression of VEGF-C and VEGF-D in pig, rabbit and mouse skeletal muscles. Here we have used the activated mature forms of VEGF-C and VEGF-D expressed via recombinant adeno-associated virus (rAAV), which provides stable, long-lasting transgene expression in various tissues including skeletal muscle. Mouse tibialis anterior muscle was transduced with rAAV encoding human or mouse VEGF-C or VEGF-D. Two weeks later, immunohistochemical analysis showed increased numbers of both blood and lymph vessels, and doppler ultrasound analysis indicated increased blood vessel perfusion. The lymphatic vessels further increased at the four-week time point were functional, as shown by FITC-lectin uptake and transport. Furthermore, receptor activation and arteriogenic activity were increased by an alanine substitution mutant of human VEGF-C (C137A) having an increased dimer stability and by a chimeric CAC growth factor that contained the VEGF receptor-binding domain flanked by VEGF-C propeptides, but only the latter promoted significantly more blood vessel perfusion when compared to the other growth factors studied. We conclude that long-term expression of VEGF-C and VEGF-D in skeletal muscle results in the generation of new functional blood and lymphatic vessels. The therapeutic value of intramuscular lymph vessels in draining tissue edema and lymphedema can now be evaluated using this model system.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776655

DOI: 10.1161/CIRCRESAHA.109.197830
PubMed: 19443835
PubMed Central: 2776655

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle</title>
<author><name sortKey="Anisimov, Andrey" sort="Anisimov, Andrey" uniqKey="Anisimov A" first="Andrey" last="Anisimov">Andrey Anisimov</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Korpisalo, Petra" sort="Korpisalo, Petra" uniqKey="Korpisalo P" first="Petra" last="Korpisalo">Petra Korpisalo</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Soronen, Jarkko" sort="Soronen, Jarkko" uniqKey="Soronen J" first="Jarkko" last="Soronen">Jarkko Soronen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Kaijalainen, Seppo" sort="Kaijalainen, Seppo" uniqKey="Kaijalainen S" first="Seppo" last="Kaijalainen">Seppo Kaijalainen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Lepp Nen, Veli Matti" sort="Lepp Nen, Veli Matti" uniqKey="Lepp Nen V" first="Veli-Matti" last="Lepp Nen">Veli-Matti Lepp Nen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Jeltsch, Michael" sort="Jeltsch, Michael" uniqKey="Jeltsch M" first="Michael" last="Jeltsch">Michael Jeltsch</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Yl Herttuala, Seppo" sort="Yl Herttuala, Seppo" uniqKey="Yl Herttuala S" first="Seppo" last="Yl Herttuala">Seppo Yl Herttuala</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">19443835</idno>
<idno type="pmc">2776655</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776655</idno>
<idno type="RBID">PMC:2776655</idno>
<idno type="doi">10.1161/CIRCRESAHA.109.197830</idno>
<date when="2009">2009</date>
<idno type="wicri:Area/Pmc/Corpus">002736</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002736</idno>
<idno type="wicri:Area/Pmc/Curation">002735</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002735</idno>
<idno type="wicri:Area/Pmc/Checkpoint">003828</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003828</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">002E66</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002E66</idno>
<idno type="wicri:Area/PubMed/Curation">002E66</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002E66</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002E66</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002E66</idno>
<idno type="wicri:Area/Ncbi/Merge">003409</idno>
<idno type="wicri:Area/Ncbi/Curation">003409</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003409</idno>
<idno type="wicri:doubleKey">0009-7330:2009:Anisimov A:activated:forms:of</idno>
<idno type="wicri:Area/Main/Merge">006493</idno>
<idno type="wicri:Area/Main/Curation">006415</idno>
<idno type="wicri:Area/Main/Exploration">006415</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle</title>
<author><name sortKey="Anisimov, Andrey" sort="Anisimov, Andrey" uniqKey="Anisimov A" first="Andrey" last="Anisimov">Andrey Anisimov</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Korpisalo, Petra" sort="Korpisalo, Petra" uniqKey="Korpisalo P" first="Petra" last="Korpisalo">Petra Korpisalo</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Soronen, Jarkko" sort="Soronen, Jarkko" uniqKey="Soronen J" first="Jarkko" last="Soronen">Jarkko Soronen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Kaijalainen, Seppo" sort="Kaijalainen, Seppo" uniqKey="Kaijalainen S" first="Seppo" last="Kaijalainen">Seppo Kaijalainen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Lepp Nen, Veli Matti" sort="Lepp Nen, Veli Matti" uniqKey="Lepp Nen V" first="Veli-Matti" last="Lepp Nen">Veli-Matti Lepp Nen</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Jeltsch, Michael" sort="Jeltsch, Michael" uniqKey="Jeltsch M" first="Michael" last="Jeltsch">Michael Jeltsch</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
<author><name sortKey="Yl Herttuala, Seppo" sort="Yl Herttuala, Seppo" uniqKey="Yl Herttuala S" first="Seppo" last="Yl Herttuala">Seppo Yl Herttuala</name>
<affiliation wicri:level="1"><nlm:aff id="A2"> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O.B. 1627, 70211 Kuopio</wicri:regionArea>
<wicri:noRegion>70211 Kuopio</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<affiliation wicri:level="4"><nlm:aff id="A1"> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki, Finland</nlm:aff>
<country xml:lang="fr">Finlande</country>
<wicri:regionArea> Molecular/Cancer Biology Laboratory, Biomedicum Helsinki, Department of Pathology, Haartman Institute and Helsinki University Central Hospital, P.O.B. 63, (Haartmaninkatu 8), 00014 University of Helsinki</wicri:regionArea>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
<orgName type="university">Université d'Helsinki</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">Circulation research</title>
<idno type="ISSN">0009-7330</idno>
<idno type="eISSN">1524-4571</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Blood Vessels (physiology)</term>
<term>Dimerization</term>
<term>Drug Stability</term>
<term>Heart (physiology)</term>
<term>Humans</term>
<term>Lymphatic Vessels (physiology)</term>
<term>Mice</term>
<term>Mice, Transgenic</term>
<term>Muscle, Skeletal (blood supply)</term>
<term>Muscle, Skeletal (physiology)</term>
<term>Mutation</term>
<term>Polymorphism, Single Nucleotide</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Vascular Endothelial Growth Factor B (genetics)</term>
<term>Vascular Endothelial Growth Factor B (physiology)</term>
<term>Vascular Endothelial Growth Factor C (genetics)</term>
<term>Vascular Endothelial Growth Factor C (physiology)</term>
<term>Vascular Endothelial Growth Factor Receptor-2 (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Coeur (physiologie)</term>
<term>Dimérisation</term>
<term>Facteur de croissance endothéliale vasculaire de type B (génétique)</term>
<term>Facteur de croissance endothéliale vasculaire de type B (physiologie)</term>
<term>Facteur de croissance endothéliale vasculaire de type C (génétique)</term>
<term>Facteur de croissance endothéliale vasculaire de type C (physiologie)</term>
<term>Humains</term>
<term>Muscles squelettiques ()</term>
<term>Muscles squelettiques (physiologie)</term>
<term>Mutation</term>
<term>Polymorphisme de nucléotide simple</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Récepteur-2 au facteur croissance endothéliale vasculaire (physiologie)</term>
<term>Souris</term>
<term>Souris transgéniques</term>
<term>Stabilité de médicament</term>
<term>Vaisseaux lymphatiques (physiologie)</term>
<term>Vaisseaux sanguins (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Vascular Endothelial Growth Factor B</term>
<term>Vascular Endothelial Growth Factor C</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Muscle, Skeletal</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteur de croissance endothéliale vasculaire de type B</term>
<term>Facteur de croissance endothéliale vasculaire de type C</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coeur</term>
<term>Facteur de croissance endothéliale vasculaire de type B</term>
<term>Facteur de croissance endothéliale vasculaire de type C</term>
<term>Muscles squelettiques</term>
<term>Récepteur-2 au facteur croissance endothéliale vasculaire</term>
<term>Vaisseaux lymphatiques</term>
<term>Vaisseaux sanguins</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Blood Vessels</term>
<term>Heart</term>
<term>Lymphatic Vessels</term>
<term>Muscle, Skeletal</term>
<term>Vascular Endothelial Growth Factor B</term>
<term>Vascular Endothelial Growth Factor C</term>
<term>Vascular Endothelial Growth Factor Receptor-2</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Dimerization</term>
<term>Drug Stability</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Transgenic</term>
<term>Mutation</term>
<term>Polymorphism, Single Nucleotide</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Dimérisation</term>
<term>Humains</term>
<term>Muscles squelettiques</term>
<term>Mutation</term>
<term>Polymorphisme de nucléotide simple</term>
<term>Souris</term>
<term>Souris transgéniques</term>
<term>Stabilité de médicament</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">The therapeutic potential of vascular endothelial growth factor C (VEGF-C) and VEGF-D in skeletal muscle has been of considerable interest as these factors have both angiogenic and lymphangiogenic activities. Previous studies have mainly employed adenoviral gene delivery for short-term expression of VEGF-C and VEGF-D in pig, rabbit and mouse skeletal muscles. Here we have used the activated mature forms of VEGF-C and VEGF-D expressed via recombinant adeno-associated virus (rAAV), which provides stable, long-lasting transgene expression in various tissues including skeletal muscle. Mouse tibialis anterior muscle was transduced with rAAV encoding human or mouse VEGF-C or VEGF-D. Two weeks later, immunohistochemical analysis showed increased numbers of both blood and lymph vessels, and doppler ultrasound analysis indicated increased blood vessel perfusion. The lymphatic vessels further increased at the four-week time point were functional, as shown by FITC-lectin uptake and transport. Furthermore, receptor activation and arteriogenic activity were increased by an alanine substitution mutant of human VEGF-C (C137A) having an increased dimer stability and by a chimeric CAC growth factor that contained the VEGF receptor-binding domain flanked by VEGF-C propeptides, but only the latter promoted significantly more blood vessel perfusion when compared to the other growth factors studied. We conclude that long-term expression of VEGF-C and VEGF-D in skeletal muscle results in the generation of new functional blood and lymphatic vessels. The therapeutic value of intramuscular lymph vessels in draining tissue edema and lymphedema can now be evaluated using this model system.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Finlande</li>
</country>
<region><li>Uusimaa</li>
</region>
<settlement><li>Helsinki</li>
</settlement>
<orgName><li>Université d'Helsinki</li>
</orgName>
</list>
<tree><country name="Finlande"><region name="Uusimaa"><name sortKey="Anisimov, Andrey" sort="Anisimov, Andrey" uniqKey="Anisimov A" first="Andrey" last="Anisimov">Andrey Anisimov</name>
</region>
<name sortKey="Alitalo, Annamari" sort="Alitalo, Annamari" uniqKey="Alitalo A" first="Annamari" last="Alitalo">Annamari Alitalo</name>
<name sortKey="Alitalo, Kari" sort="Alitalo, Kari" uniqKey="Alitalo K" first="Kari" last="Alitalo">Kari Alitalo</name>
<name sortKey="Jeltsch, Michael" sort="Jeltsch, Michael" uniqKey="Jeltsch M" first="Michael" last="Jeltsch">Michael Jeltsch</name>
<name sortKey="Kaijalainen, Seppo" sort="Kaijalainen, Seppo" uniqKey="Kaijalainen S" first="Seppo" last="Kaijalainen">Seppo Kaijalainen</name>
<name sortKey="Korpisalo, Petra" sort="Korpisalo, Petra" uniqKey="Korpisalo P" first="Petra" last="Korpisalo">Petra Korpisalo</name>
<name sortKey="Lepp Nen, Veli Matti" sort="Lepp Nen, Veli Matti" uniqKey="Lepp Nen V" first="Veli-Matti" last="Lepp Nen">Veli-Matti Lepp Nen</name>
<name sortKey="Soronen, Jarkko" sort="Soronen, Jarkko" uniqKey="Soronen J" first="Jarkko" last="Soronen">Jarkko Soronen</name>
<name sortKey="Yl Herttuala, Seppo" sort="Yl Herttuala, Seppo" uniqKey="Yl Herttuala S" first="Seppo" last="Yl Herttuala">Seppo Yl Herttuala</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006415 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006415 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:2776655
   |texte=   Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:19443835" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

Serveur d'exploration sur le lymphœdème

Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle

Activated forms of VEGF-C and VEGF-D provide improved vascular function in skeletal muscle

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.